Вплив вінборону на аналгетичну активність ібупрофену на моделі ад’ювантного артриту у щурів

Abstract

Експериментально встановлено, що вінборон здатен потенціювати аналгетичну активність ібупрофену при лікуванні ад'ювантного артриту (АА) у щурів. На це вказувало зростання порогу больової чутливості на 28 добу експерименту при лікуванні АА комбінацією ібупрофену з вінбороном, який вдвічі перевищував аналогічний показник при монотерапії ібупрофеном, Крім того, вінборон показав спроможність послаблювати ульцерогенний ефект ібупрофену, про що свідчили динаміка маси тіла, виразковий індекс та відсутність летальності тварин. /// Ibuprofen - NSAIDs, which are widely used in the treatment of various chronic inflammatory and degenerative diseases of the musculoskeletal system. In our opinion, the combined use of ibuprofen and vinboron - new domestic antispasmodics with polytropic pharmacological effects will not only improve the safety profile of said NSAIDs but have favorable effects on comorbid patient background. The aim describe the impact on vinboronu analgesic effect of ibuprofen on the model of adjuvant arthritis (AA) in rats. Materials and methods. The survey was conducted on 28 mature male rats, divided into 4 groups: I - intact rats (n = 7), II - rats with simulated AA untreated (control), III - rats with AA (n = 5-7), treated ibuprofen (218 mg / kg, intragastric). IV- rats with AA (n = 7) treated with ibuprofen w / w (218 mg / kg) in combination with vinboronom (11 mg / kg, intragastric). Vinboron dissolved 0.9% solution of NaCl, was administered for 60 minutes. the introduction of ibuprofen. Ibuprofen was administered as a suspension intragastric 3% starch mucus twice daily (109 mg / kg at one time). Conversion of drugs with human dose on rats performed using species sensitivity factor Y. R. Rybolovlev. AA modeled by introducing subplan complete Freund's adjuvant (Imject Freund's Complete Adjuvant, Thermo Scientific) in the back right leg at the rate of 0.1 ml per rat ("Preclinical studies", ed. A. Stefanova, 2002). Day introduction of adjuvant considered as "0" day of the experiment. The maximum expression of the local inflammatory response, which was accompanied by a significant increase in limb volume was estimated at 12-14 days, then gradually decreased activity factors. Treatment was conducted from 14 to 28 days AA by intragastric administration of study drug. Beginning of the introduction of drugs corresponded maximum inflammatory response. Evaluating the effectiveness of treatment was performed on day 28 of the experiment. Results and discussion. Results of the study showed that administration of adjuvant control group animals resulted in statistically significant reduction pain sensitivity relatively intact and original values, namely 18.6%. A slight reduction in pain sensitivity (7.4%) in this group of animals was noted on day 28 relative to day 14 of the experiment. A similar pattern is 14 days of the experiment was determined in the third group of animals (AA treated ibuprofen), where the rate of pain sensation statistically significantly decreased relative to the initial rate of 17%. However, treatment of the third group of animals, ranging from 14 days ibuprofen resulted in a statistically significant increase in pain threshold by 16% relative to the initial level consistent with the literature on distinct analgesic activity of ibuprofen in the treatment of chronic degenerative diseases of the connective tissue. The most pronounced changes in pain sensitivity were recorded in the group of animals treated with 14 days of the experiment with a combination of ibuprofen vinboron. This indicated statistically significant increase in pain sensitivity by 2 times, namely by 39,2% compared to the initial rate. This shows the ability vinboronu potentiate the analgesic effect of ibuprofen by known him, according to the literature, analgesic, antispasmodic and anti-inflammatory activity Conclusions: vinboron increases analgesic activity of ibuprofen in the treatment of experimental adjuvant arthritis (AA). This indicated increase in pain threshold on day 28 of the experiment in the treatment of AA combination with ibuprofen vinboronom which twice exceeded the monotherapy, respectively +15,9% and +39,2%. In addition vinboron showed the ability to weaken the ulcerogenic effect of ibuprofen, as evidenced by the dynamics of body mass index and mortality ulcerative animals.