Abstract:
Чиж МО, Матвєєнко МС, Гладких ФВ, Лядова ТІ, Коморовський РР, Козлова ТВ. Оцінка кардіопротектор¬ної активності кріоекстракту серця на моделі адреналінової міокардіодистрофії за показниками вільноради¬кального окиснення. The Journal of V.N. Karazin Kharkiv National University. Series Medicine. 2025;33. № 2 (53):178–193. DOI: https://doi.org/10.26565/2313-6693-2025-53-02
Description:
Myocardial disease typically involves pronounced oxidative stress, which contributes to cardiomyocyte damage, myocardial remodeling, and the development of heart failure. Current therapeutic approaches are not always capable of fully neutralizing the excessive production of free radicals; therefore, the search for new cardioprotective agents capable of correcting the imbalance between reactive oxygen species (ROS) and antioxidant mechanisms is highly relevant.
Purpose – To investigate the cardioprotective activity of porcine heart cryoextract, particularly its effect on oxidative stress markers in cardiac tissue and blood serum using a rat model of adrenaline-induced myocardial dystrophy (AMD).
Materials and Methods. The experiment was conducted on 84 outbred male rats divided into four groups (n = 21): intact animals; rats with AMD without treatment; rats with AMD that received intraperitoneal injections of heart cryoextract (50 µg of peptides/100 g of body weight) for 14 days; and rats with AMD treated with cordarone (10 mg/kg, intramuscularly) for 14 days. Levels of lipid peroxidation products were assessed: thiobarbituric acid reactive substances (TBARS) and diene conjugates in heart homogenates, and malondialdehyde (MDA) in blood serum on days 2, 7, and 14 after AMD induction.
Results. AMD induction was associated with a significant increase in TBARS (+140.5%), MDA (+82.5%), and diene conjugates (+252.9%) compared to intact rats (p < 0.001). On day 2, the cryoextract reduced TBARS by 33.7% (p < 0.001), MDA by 11.0%, and diene conjugates by 16.7%, which was comparable to the effects of cordarone (30.3% and 6.4%, respectively; p > 0.05). During the 7–14 days of treatment, both agents maintained a significant reduction in oxidative stress markers, although MDA and diene conjugate levels did not reach the baseline values observed in intact animals.
Conclusions. Porcine heart cryoextract demonstrated significant cardioprotective effects by correcting oxidative imbalance in a model of adrenaline-induced myocardial dystrophy and showed efficacy comparable to that of cordarone in reducing key markers of free radical oxidation. Heart cryoextract appears to be a promising agent in the pathogenetic treatment of AMD, aimed at lowering oxidative stress, stabilizing membranes, restoring antioxidant enzyme activity, and preserving redox homeostasis
